Question to be addressed Electroporation, lipid transfection, and biolistic particle delivery systems have been used to deliver genetic material into cells but either are unsuitable for delivery to mammals; or there is a lack of success in delivering the DNA material intact to the target cells. It should be possible to design a nanoparticle-containing genetic coding as plasmid DNA for intracellular delivery. The design and assembly of such nano-structure can be achieved utilizing methods currently used in structural DNA nanotechnology and constitutes an exciting new area of development in Pharmaceutical Biotechnology. The aim of this study is to utilize structural DNA technology methods for the design of a nano-architecture using a double stranded DNA plasmid. In order to achieve this goal the Project Proposal will contain two main features: (i) A review of the literature should allow for the selection of a currently available plasmid that encodes for a fluorescent protein. The fluorescent protein will allow for assessing intracellular delivery and functionality of the plasmid. (ii) The proposal should contain the principles of DNA nanotechnology that will be employed for the design of the nanoparticle. The objective of the Project In this project our aim is to design a nanoparticle containing a DNA plasmid with suitable dimensions for delivery into cells. Methods Computer-based project using: – Cadnano (cadnano.org) – Vector diagrams using Inkscape (inkscape.org/en) – AutoCAD Maya Expected outcome(s) Plasmid and list of DNA staples, along with their organization (vector diagrams, graphs, illustrations). References – Donnelly JJ, Wahren B, Liu MA. DNA vaccines: Progress and challenges. J Immunol 2005; 175(2): 633-639. – Lin C, Liu Y, Yan H. Designer DNA Nanoarchitectures. Biochemistry (N.Y.) 2009; 48(8): 1663-1674. – Dietz H, Douglas SM, Shih WM. Folding DNA into Twisted and Curved Nanoscale Shapes. Science 2009; 325(5941): 725-730
Try it now!
How it works?
Follow these simple steps to get your paper done
Place your order
Fill in the order form and provide all details of your assignment.
Proceed with the payment
Choose the payment system that suits you most.
Receive the final file
Once your paper is ready, we will email it to you.
Our Services
Custom Writings Help is a Quality-Oriented Company in Online Writing as a result of hiring exceptional professionals to execute clients' tasks.
Essays
At Custom Writings Help,We understand the struggle of research paper writing, and that is why at Custom WritingS Help, we are all out to help you. We pride ourselves on having a team of clinical writers. The stringent and rigorous vetting process ensures that only the 'BEST' Writers are chosen for the job. We have highly qualified Ph.D. and MA writers working with us; we equally offer these experienced writers specific bonuses and incentives to make them deliver highly original, unique, and informative content at reasonably low prices.
Admissions
Thesis Writing Service
Worlwide, Many Masters Students are struggling with Thesis Completion. A thesis is likely to be the longest and most challenging piece of work a student has ever completed. However, unlike essays and other assignments, a student can pick a particular interest topic and work on their initiative. Fortunately, we are there for you. At Custom Writings Help, you are assured of an authentic, imaginative, informative, linguistically great, and advantageous thesis that adheres to all your needs. So, why continue considering different writers when you have discovered the best in the field?
Editing
Custom Papers
Not a single student can avoid writing custom papers. However, a total lack of experience, skills, and time makes it very hard to produce a superb writing piece. Therefore, if you are seeking professional help, turn to us. Our specialized and experienced writers compose a variety of model papers, including custom essays, college term papers, research papers, book reports, MBA essays, executive summaries, dissertations, Ph.D. theses, admission essays, and research proposals for college and university students at any level.
Coursework
Essay Writing
Most of the students disregard the critical principles of essay writing and compose papers below sensible guidelines. Therefore, with Custom Writings Help, one should not worry about his/her essay. Our Writers compose informative and engaging content on all complexities and topics. We write meaningful and smart essays while prioritizing all aspects that bring about a good grade, such as impeccable grammar, proper structure, zero-plagiarism, and conformance to guidelines.
Coursework
Coourse Work Writing
Don't let the seemingly never-ending onslaught of writing assignments get you down. If you are looking where to get course work assistance online, the writers at Custom Writings Help are here to assist you with all of your writing needs. We undertake to unique delivery of papers that meet the professor's requirements. The content is proofread, edited, and checked plagiarism before submission to customers. No matter how big or small your work is, we will deliver on time. Try US Now! !
Coursework
Dissertation Writing Service
High-Quality Dissertation Writing Services are rare. They require Ph.D. academicians – not easily found. However, are an exception. The years, time, and resources we have invested in the dissertation world has given us a competitive advantage over others. Choose to come to Custom Writings Help; You will find perfect Ph.D. consultants who have written hundreds of dissertations theses ready to help you. Let our dissertation-writing services help you craft your dissertation, for you are assured we will give you the results.