You should first analyse the data you are provided with to determine the amount of
IL-6 and TNFa produced by each of the four experimental samples (A-D).
a) You should plot and present annotated standard curves of the two ELISA
experiments on graphs using an appropriate analysis package (e.g. Excel) – ensure
graphs are presented and labelled appropriately. (8 marks)
b) You should then calculate the concentrations of cytokines secreted in your
unknown samples (A-D) and complete data tables D & E. Present the results of your
analysis of ALL the experimental samples (including those generated above) in
separate graphs showing the degree of biological variation. (6 marks)
c) You should describe your results (500 words maximum). Remember to
include a description of what your CLP samples did in comparison to respective
controls and the differences between the samples treated with the
immunomodulatory agent compared to controls. You should comment on what the
sensitivity of the two ELISA experiments was and how reliable and reproducible your
results are. (16 marks)
d) You should then give a brief discussion and conclusion for your findings
(500 words maximum). Why were these two cytokines measured? What can they tell
us about the immune response in sepsis? Why might pre-treatment with the
immunomodulatory agent aid recovery from sepsis in humans and what cells and
immune pathways might it be acting upon. Finally, can you find any research
literature that supports these conclusions in human disease? (20 marks)
The deadline for submission of the coursework is 4pm Thursday 21st November.
The coursework should be submitted online via Keats.
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